ASCP MLS Route 6: Eligibility Requirement + Common Documentation
The American Society for Clinical Pathology Board of Certification (ASCP BOC) specific series of MLS ASCP routes for medical lab scientists from MLS ASCP Route 1 – ASCP MLS Route 6.
Some eligibility prerequisites that are common in all routes (ASCP MLS Route 6, etc, and others) are
Academic educational degrees (baccalaureate or master’s degree), training programs that must be obtained from approved institutions, & relevant field clinical experience from accredited labs.
Through these eligibility paths, the board chooses the right candidates for lab experiments.
6 routes are fixed for United States candidates and 5 routes for international candidates by the Board of Certification.
It is not necessary to meet all route requirements, satisfying only one route requirement is enough.
Here, we are going to concentrate on the ASCP MLS Route 6 requirement: You will find all the necessary, detailed information here.
All That You Need to Know: ASCP MLS Route 6 Overview
The following is a detailed description of ASCP MLS Route 6 criteria:
- Medical laboratory training course: must be of 50 weeks long and sponsored by the U.S. military (must be obtained within last 10 years)
- A bachelor’s degree with a combination of 16 semester hours in chemistry & bio with 1 semester hrs in microbiology, must be obtained from an accredited institution.
- Clinical experience in microbiology, hematology, chemistry, urinalysis & body fluids, and immunology from an acceptable lab: This experience must be one year
Authorized College & University
Approved colleges or universities in the United States are listed by the USDE
- USDE- United States Department of Education
Approved colleges or universities in Canada are listed by the following association:
- AUCC-Association of Universities and Colleges of Canada
The lab that you chose to get the lab experience must be approved by JCI accreditation, DoD CLIP certificate of registration, compliance, accreditation, accreditation under ISO 15189, or CMS CLIP certificate of registration, compliance, accreditation.
Special Note
Make sure to upload the following documentation in PDF format:
- Official copy of your ER.B, or DD214
- Official certificate of training verification
- Official documentation of your military code ( 68K, AFSC code, MOS, NEC)
Common Documentation for ASCP MLS Route 6
With the verification of ASCP MLS route 6, you have to meet some common requirements that are given below:
- For education within the U.S. or Canada; you require an official transcript.
- For education outside of the U.S. or Canada; (acceptable) official transcript evaluation.
- Letter of authenticity with experience documentation form
- Letter of authenticity with training documentation form
- Military training official certificate or notarized
If you realize that you are not eligible for the ASCP MLS Route 6 requirements, you may consider the following routes:
- ASCP MLS Route 1
- ASCP MLS Route 2
- ASCP MLS Route 3
- ASCP MLS Route 4
- ASCP MLS Route 5
How to Submit the Documentation to Complete Application Form
You cannot complete your application form without uploading all the documentation accurately.
- Make sure to upload all the documents in PDF style: Pictures, etc are not allowed.
- The size of the file must be less than 10 MB.
- No electronic signatures are accepted; All the signatures must be original & handwritten
- An official transcript must be sent by your educational institution directly to ASCP BOC.
- Never send your letter of authenticity or documentation form via mail, it will not be accepted
FAQs for ASCP MLS Route 6
What are the eligibility conditions for ASCP MLS Route 6?
A bachelor’s degree with a combination of 16 semester hrs in biology & chemistry, 50 weeks of medical lab training course by the U.S. military, and relevant subject clinical experience (must be of one year).
For MLS ASCP/ASCPi, how many routes are fixed by the board?
6 routes for MLS ASCP (U.S. candidates) and 5 routes for MLS ASCPi (international candidates) are fixed by the board.
Which common documentation must I have to meet eligibility for MLS ASCP?
Official transcript (education within the U.S. or Canada), transcript evaluation (education outside of the U.S.), military training official certificate, and training & experience documentation with a letter of authenticity.
Resources: ASCP BOC.

Abdelhalim Elshawadfy is an ASCP certified Medical Laboratory Scientist (MLS ASCP), Specialist in Microbiology (SM ASCP), and Molecular Biology Technologist (MB ASCP) with a distinguished career marked by expertise, continuous learning, and a commitment to elevating the standards of clinical laboratory science. As an ASCP Certification Expert and American Board Certification Expert, Abdelhalim Elshawadfy continues to make invaluable contributions to the field, leaving a lasting impact on the medical community.
