MB (ASCP) – Technologist in Molecular Biology

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MB ASCP technologist is one of two unique certificates in molecular biology for medical laboratory technologists skilled in DNA and RNA testing. It's awarded by the American Society for Clinical Pathology Board of Certification, ASCP BOC.

Moreover, it is also for those who want to advance their career in the field of molecular biology, genetics and biotechnology to get nominated and certified as MB (ASCP/ ASCPi)CM by the most prestigious organization in the field of clinical pathology, ASCP.

This American Society for Clinical Pathology BOC certification is also suitable for those who want to learn novel techniques or upgrade their career.

However, it is also for international medical technologists who are preparing to get work licensure in many countries especially in the USA.

Watch: MB (ASCP) – Technologist in Molecular Biology Overveiw

Here is the quick video, breaking down MB (ASCP) Credential. Everything you need to know about technologist in molecular biology certification by ASCP. If you prefer watching over reading, then start here?

What is the MB (ASCP)CM?

MB (ASCP) is a certification awarded to technologists in molecular biology by ASCP. Moreover, the demand for molecular biologists is increasing. So, the American Society for Clinical Pathology offers ASCP Molecular Biology certification.

MB ASCP Exam Prep Course

ASCP Molecular Biology Technologists are responsible for diagnosing diseases through biological methods. They also use advanced techniques to help identify the most effective treatments. However, this career is rewarding and challenging.

Requirements for MB (ASCP) Technologist in Molecular Biology

Firstly, make sure that you are eligible for the ASCP exam per the American Society for Clinical Pathology (BOC) before applying, as the application fee is non-refundable.

The application fee for the MB is $200, though it may be around $95 for some countries.

There are four eligibility routes for MB certification. You must meet all the requirements of at least one route.

US Routes for MB ASCP

Route 1

  • ASCP Technologist/Scientist or Specialist certification
  • A bachelor’s degree from a regionally accredited college or university

Route 2

  • A bachelor’s degree from a regionally accredited college or university
  • Completion of a NAACLS-accredited Diagnostic Molecular Science program within the last five years

Route 3

  • A bachelor’s degree from a regionally accredited college or university with a major in biological science or chemistry
    OR
  • A bachelor’s degree from a regionally accredited college or university with a combination of 30 semester hours in biology and chemistry
  • One year of clinical experience in a molecular biology lab within the last five years
    OR
  • One year of veterinary, industry, or research experience in a molecular biology lab in the U.S. or Canada within the last five years

Route 4

  • A master’s degree from a regionally accredited college or university in molecular biology, biotechnology, chemistry, biology, immunology, microbiology, allied health, medical laboratory science, or a related field
  • Six months of clinical experience in a molecular biology lab within the last five years
    OR
  • Six months of veterinary, industry, or research experience in a molecular biology lab in the U.S. or Canada within the last five years.

International Routes for MB(ASCP)

Route 1

  • A valid ASCPi technologist, scientist, or specialist certification, and have a bachelor’s degree from an accredited or approved school.

Route 2

  • A bachelor’s degree from an accredited or approved institution, plus you must complete an approved diagnostic molecular science program that includes an internship. Your academic or program coursework must cover biology and chemistry.

Route 3

  • A bachelor’s degree from an accredited or approved school qualifies you.

Route 4

  • A bachelor’s degree in biological science or chemistry, or a bachelor’s with at least 30 semester hours combined in biology and chemistry (either as part of your degree or as additional coursework). 
  • One year of clinical experience in a molecular biology lab, or one year of relevant experience in a veterinary, research, or industry setting.

Route 5

  • A master’s degree in molecular biology, chemistry, biology, immunology, microbiology, medical laboratory science, or a closely related field. 
  • Six months of hands-on experience in a molecular biology lab, either clinical, veterinary, industry, or research-based.

Molecular Biology Laboratory

A molecular biology laboratory provides individuals with knowledge and hands-on experience in molecular techniques. It includes nucleic acid isolation, amplification, detection, sequencing, hybridization methods, and data analysis.

MB ASCP Exam Prep Course

Eligibility Check for MB ASCP Technologist in Molecular Biology

Use the BOC Examination Eligibility Assistant for MB (ASCP). It helps you determine if you qualify for the American Society for Clinical Pathology BOC certification. The process includes the following steps:

  • Accept the terms and conditions
  • Select your certificate type
  • Fill out the questionnaire
  • View your eligibility result

Follow these steps to check your eligibility. In this example, we’re using Route 1 as the reference.

  1. Eligibility Assistant for MB ASCP Route Determination

Firstly, go to eligibility assistant of the American Society For Clinical Pathology BOC . Secondly, select the checkbox to agree with ASCP BOC terms. Then, click on begin.

1 MB ASCP Eligability Check
  1. MB ASCP Route Determination: Select certificate

Then, select the certificate from the list of ASCP certification programs.

2 1 Select Certificate
  1. Check MB – Technologist in Molecular Biology

For MB ASCP, check the MB – Technologist in Molecular Biology. You will see it under the additional certifications section. Finally, click on next.

2 2 Technologist in Molecular Biology Selected
  1. Fill Questionnaire

After that, a questionnaire will pop up. Certainly, the questions will be related to your education and experience. Firstly, select your desired option. Then, hit next to move forward.

Furthermore, you can also view the progress bar at the bottom. However, you can return to any question at any time by clicking on the previous button.

  • Question 1: Do you have a bachelor's degree from a regionally accredited college or university?
ASCP Study Material
  • Question 2: Have you successfully completed a NAACLS* accredited diagnostic molecular science program within the last five years?
4 Completed NAACLS accredited diagnostic molecular Prog
  • Question 3: Do you have a valid (not expired) ASCP certification as a technologist/scientist or specialist?
5 Have a valid ASCP certificate
  1. Eligibility Results

Finally, you can view the eligibility results. In this example, we used Route 1 for MB (ASCP), so you’ll see the specific requirements listed for that route. If your inputs meet those criteria, it means you fulfill the Route 1 requirements for ASCP Molecular Biology Certification.

How to Pass M ASCP Exam

You can find the Eligibility Checker here .

MB ASCP Exam Prep Course

MB (ASCP)Exam Questions, Pass Score, Study Guide, and Material

The ASCP Molecular Biology Certification exam includes 100 questions, and the time limit is 2 hours and 30 minutes. The test is tailored to match each individual's ability level.

It uses computer adaptive testing (CAT), meaning the difficulty changes based on your answers. If you get a question right, the next one will be harder. If you miss one, the next will be easier.

Each question is weighted based on its difficulty. The passing score is 400. To earn your ASCP Molecular Biology Technologist certification, you’ll need to answer as many questions correctly as possible.

Looking for an MB (ASCP)study guide and materials? Keep reading.

ASCP Exam MLSiAcademy

MB ASCP Exam Study Guide Per BOC Site

The exam questions comprise four subjects. Moreover, subject names and percentages are as follows:

  • Applications of Molecular Testing: 30–35%
  • Molecular Techniques: 30–35%
  • Molecular Science: 20–25%
  • Lab Operations: 15–20%
MB ASCP Technologist Molecular Biology Content

Molecular Science

The molecular science contents for ASCP Molecular Biology Certification are:

  • Basic molecular theory
  • Chemistry of Nucleic acid
  • Human/microbial genetics
  • Biochemical reagents

Molecular Techniques

The Molecular Techniques contents for Molecular Biology Certification by ASCP are:

  • Nucleic acid (NA) isolation
  • NA separation and detection
  • NA amplification
  • DNA sequencing
  • Other molecular techniques

Lab Operations

The Lab Operations section includes:

  • Specimen processing
  • Contamination control
  • Reagents
  • Assays (performance, validation, and troubleshooting)
  • Proficiency testing
  • Results (calculation, interpretation, and reporting)
  • Instrumentation
  • Guidelines and regulations
  • Safety
  • Continuing education
  • Quality control (QC)
  • Competency assessment

Applications of Molecular Testing

This section includes:

  • Genetic disorders
  • Genetic identity
  • Infectious diseases
  • Pharmacogenomics
  • Histocompatibility
  • Oncology
  • Bone engraftment

MB ASCP Exam Reading List Acc. To ASCP BOC Site

For ASCP Molecular Biology Technologist Certification, you can refer to the following textbooks as seen on the ASCP site.

General Molecular Biology

  • Molecular Biology of the Cell (6th ed.). New York: Garland Science.
  • Molecular Diagnostics: Fundamentals, Methods, & Clinical Applications (3rd ed.). Philadelphia: F.A. Davis Company.
  • The Molecular Basis of Human Disease (2 nd ed.). Elsevier Inc.
  • The Cell: A Molecular Approach (8th ed.). New York, NY: Oxford University Press.
  • Lewin's Genes XII (12th ed.). Burlington, MA: Jones & Bartlett Publishers. 
  • Principles and Applications of Molecular Diagnostics. Elsevier Inc.
American Society For Clinical Pathology Board Of Certification (ASCP BOC).

Applications Of Molecular Science

  • Diagnostic Molecular Pathology: A Guide to Applied Molecular Testing. Elsevier Inc.
  • Principles and Practice of Oncology: Primer of the Molecular Biology of Cancer (3rd Ed.). Philadelphia: Wolters Kluwer Health.
  • Hematopathology – CHAPTER 6: Molecular Diagnosis in Hematopathology ONLY (2nd ed.). Philadelphia, PA: Elsevier, Inc.
  • Clinical Genomics: A Guide to Clinical Next Generation Sequencing. Elsevier Inc. 
  • Molecular Pathology in Clinical Practice (2nd ed.). New York: Springer. 
  • Thompson and Thompson Genetics in Medicine (8th ed.). Philadelphia, PA: Elsevier Inc. 
  • Molecular Microbiology: Diagnostic Principles and Practice (3rd ed.). Washington D.C.: ASM Press.
American Society For Clinical Pathology Board Of Certification (ASCP BOC).

Mathematics

  • Fundamental Laboratory Mathematics: Required Calculations for the Medical Laboratory Professional. Philadelphia, PA: F.A. Davis Company.
  • Mathematics for the Clinical Laboratory (4th ed.). St. Louis: Elsevier.
  • Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory (3rd ed.). London: Academic Press.
ASCP Board Of Certification (ASCP BOC).

You can also access online content for Molecular Biology Technologist certification from the following associations recommended by the American Society for Clinical Pathology (BOC):

  • Association for Molecular Pathology (AMP
  • Association of Genetic Technologists (AGT
  • American Association for the Study of Liver Disease (AASLD
  • American Society for Histocompatibility and Immunogenetics (ASHI
  • Clinical and Laboratory Standards Institute (CLSI
  • American Society for Microbiology (ASM
  • College of American Pathologists (CAP
  • Pan American Society for Clinical Virology (PASCV)
  • National Center for Biotechnology Information (NCBI)

But if your are looking for a shortcut? For a comperhenisve MB study material, exam questions and online classes, Check our MB ASCP Prep Course?

MB (ASCP)Exam Prep: The All-in-One Solution

Looking for the right ASCP MB exam prep?

The ASCP Technologist in Molecular Biology exam doesn’t have to feel impossible, not when you’ve got the MLSIA 4×4 MB (ASCP) exam prep review course package. This all-in-one prep solution includes live online classes, unified study material, and detailed Q&A support to help you pass with confidence.

MB ASCP Exam Prep Course

How to Get Certified in 6 Steps

So, you've decided to apply for the ASCP Molecular Biology certification. Here's a step-by-step guide to help you become a certified Molecular Biology Technologist.

⏱️Total Time: 1 minute to understand the steps

Step 1: Choose Your ASCP Eligibility Route

Determine which eligibility route you qualify for, then apply accordingly. Use the ASCP BOC eligibility assistant to guide you through the process.

Step 2: Evaluate Your Degree (International Applicants Only)

If you're applying from outside the U.S., get your transcripts evaluated by an agency accepted by the ASCP.
– Verify your experience letter as well.
– ASCP only accepts official documents, so gather everything in advance.

Need help? Learn how to evaluate your international degree through MLSi Academy.
How to Evaluate International Degree-in USA by the MLSi ACADEMY

Step 3: Create Your ASCP Account

3.1. Visit the official ASCP website and create an account.
3.2. Enter your full name exactly as it appears on your ID
3.3. Use an active email address—ASCP will send all updates via email.
You can update your contact info later if needed.

Step 4: Apply and Submit Your Fee

4.1. Complete your application with accurate education, certification, and experience details.
4.2. Review your entries carefully.
4.3. Submit the exam fee to finalize your application.
Want a full walkthrough? See the ASCP BOC Online Application Process.

Ascp Boc Exam Online Application Process

Step 5: Schedule Your Exam

5.1. Wait for a confirmation email from ASCP BOC with instructions for uploading documents.
5.2. Submit your evaluated transcript and experience documentation.
5.3. After review, ASCP will email your approval.
5.5. Use the link in that email to schedule your exam date within the allowed time frame.

MB (ASCP)Exam Prep Tips

The Molecular Biology certification exam isn’t easy, but you don’t have to tackle it alone.

Want to prep like a pro? Click the image below to access expert-led study material and live support.

MB ASCP Exam Prep Course

FAQs for MB (ASCP) Technologist in Molecular Biology Certification

What is an MB (ASCP)?

MB (ASCP) is a certified Molecular Biology Technologist credential issued by the American Society for Clinical Pathology (ASCP) Board of Certification (BOC). It’s designed for professionals skilled in DNA and RNA testing who pass the ASCP Molecular Biology Certification exam.

How do I get MB (ASCP) certified by the American Society for Clinical Pathology?

1. Check your eligibility for the Molecular Biology certification
2. Choose your certification route
3. Create an account on the ASCP BOC website
4. Complete the application
5. Submit the fee and all required official documents
6. Prepare for the exam

What is MB (ASCP)?

ASCP introduced the Credential Maintenance Program (CMP) to help you stay current in your field. If you complete the CMP, the “CM” suffix is added to your credential, making it MB (ASCP). If not, your certification remains as MB (ASCP).

How many questions are on the ASCP MB exam?

The exam has 100 questions for both MB and SMB (Specialist) certifications. Each question has a different weight depending on its difficulty level.

What is the passing score for the MB (ASCP) exam?

The passing score is 400. ASCP scores range from 0 to 999.

How do you become a Molecular Technologist?

You’ll need a bachelor’s degree in biology or a related life science field, plus relevant lab experience. A master’s degree and certification can further advance your career.

MB ASCP Exam Prep Course

What is a Molecular Technologist?

A Molecular Technologist studies biological activity at the molecular level. They work with DNA and RNA from humans, plants, and animals to support research, diagnosis, and treatment of diseases, typically in a lab setting.

What is a Registered Medical Technologist?

A Registered Medical Technologist performs laboratory tests on blood, tissues, and other body fluids to help diagnose conditions and monitor treatment.

Is Molecular Biology in demand?

Yes, demand has steadily grown over the past 20 years. The field supports ongoing research in genomics, diagnostics, biotechnology, and therapeutic development. There’s less competition compared to many other science-based careers.

What kind of jobs can you get with a Molecular Biology degree?

– Biotechnologist
– Embryologist
– Forensic DNA Analyst
– Molecular Genetics Technologist
– Molecular Laboratory Technologist
– Plant Geneticist
– Research Associate
– Research Technologist
– Clinical Research Assistant
– Assistant Biologist (Food Inspection Agencies)
– Teaching Assistant
– Quality Control Specialist (Biopharma)
– Medical Device Marketing Associate

Who works in a Molecular Laboratory?

Molecular Biology Technologists and Medical Laboratory Technicians handle complex testing involving nucleic acids and proteins.

How much money does a Molecular Biologist make?

The average salary is $61,257 per year in the U.S., though this varies by experience and location.

How many years does it take to become a Molecular Biology Technologist?

You can start with a bachelor’s degree in life sciences. To advance, certification like the MB (ASCP) and additional lab experience are recommended.

Where can a Molecular Biologist work?

They often work in molecular labs but can also be employed in hospitals, research institutions, pharmaceutical companies, universities, and government agencies.

What does a Molecular Biologist do on a daily basis?

They spend the first half of the day performing DNA and RNA tests, and the rest analyzing results and documenting findings.

Is a Molecular Biologist a good job?

Yes. The job outlook is strong, with growing demand and solid return on investment. However, it often requires advanced education and significant lab experience.

Is Molecular Biology Hard?

Yes, it’s a challenging field. It involves in-depth study of molecular systems, with heavy emphasis on chemistry and biology.