MLT ASCP Exam Questions for Chemistry, Hematology, Microbiology, Blood Bank, Immunology, Urine & Body Fluids and Lab Operations

MLT ASCP exam questions help the Board of Certification (BOC) accurately measure candidates’ skills and knowledge. BOC recommends MLT ASCP content guidelines and a reading list as a source of exam question preparation.

Candidates can get information through the BOC recommended reading list and content guidelines as well as some other resources including MLT ASCP certfication exam questions.

In this article, we will describe the nature of the ASCP exam, the number of questions, and some important questions for each BOC-recommended subject including lab operation, chemistry, immunology, microbiology, blood banking, urinalysis and other body fluids, and hematology.

Testing Nature of MLT ASCP Exam

MLT ASCP test is administered using the format of computer adaptive testing. It shows the 100 MCQ questions with one correct answer.

If you answer the question correctly the next question will be slightly tougher than the first one. But if you answer incorrectly, the upcoming question will be slightly easier.

How Many Questions Is the MLT ASCP Exam?

The MLT ASCP exam comprises 100 MCQs with one correct answer. BOC fixes different percentages for each subject including lab operation, chemistry, immunology, hematology, microbiology, urinalysis and other body fluids, and blood banking.

Questions Percentage Of MLT ASCP Exam

• Chemistry: 20 – 25%
• Hematology: 20 – 25%
• Microbiology: 15 – 20%
• Blood Banking: 15 – 20%
• Urinalysis and other Body Fluids: 5-10%
• Immunology: 5-10%
• Laboratory Operations: 5–10%

Chemistry Exam Questions for MLT ASCP/ ASCPi

The main topics for chemistry are Carbohydrates, toxicology, lipids, therapeutic drug monitoring, protein and other nitrogen-containing compounds, vitamins and nutrition, electrolytes, and endocrinology. Due to big syllabus of chemistry BOC opt 20 – 25% questions from this subject.

Chemistry questions for the exam:

1. : The correct name of the most anodic protein on electrophoresis at pH 8.6?

• Albumin
• Hemoglobin
• Transferrin
• None of them

2. In what method urea is converted into ammonia enzymatically?

• Diacetyl monoxime method
• Urease method
• Uricase method
• Direct method

3. Lactate + NAD pyruvate + NADH? Which enzyme catalyzes this reaction?

• Aldolase
• Ligases
• Transferases
• Isomerases

4. Creatine kinase is found in what set of organs.

• Skeletal muscle, skin and pancreas
• Heart muscle, kidney and pancreas
• Brain, liver, and skin
• Skeletal muscle, heart muscle, and brain

Microbiology Questions for MLT ASCP

Preanalytic and post-analytic procedures; analytic procedures for mycology, parasitology, virology, and mycobacteriology. 15 – 20% questions take from this content area for overall exam.

Microbiology question for MLT test

1. Cocci are bacteria with what shape?

• Rod shape
• Long shape
• Spherical shape
• Curved rod shape

2. The movement of bacteria due to a chemical stimulus is called.

• Chemotaxis
• Flagella movement
• Twitching
• Gliding

3. Gonococcal Infections across Newborns cause

• Permanent blindness
• Weakness of bone
• Effect the brain muscle
• Effect the kidney

4. : Breaks down blood clots are called

• DNAase
• NADase
• Hyaluronidase
• Streptokinase

5. The purpose of a positive coagulase test is.

• Observed clotting of plasma
• Observed clotting of blood
• Observed clotting of urea
• Observed clotting of urine

 Hematology Questions for the MLT ASCP Exam

Hematology and Hemostasis (including laboratory determinations, disease states, and physiology), laboratory testing, disease states, and physiology are important topics to cover for MLT ASCP exam questions. This content area covers 20-25% exam questions.

Question for Hematology

1. The composition of Hemoglobin A2 is

• 2 alpha and 2 delta chains
• 2 alpha and 1 gamma chains
• 1 alpha and 2 gamma chains
• 2 alpha and 3 gamma chains

2. % of Hemoglobin F that is found in adults is.

• 4%
• 3%
• 2%
• 1%

3. Reticuloendothelial cells can destroy

• Red Blood Cells
• White Blood Cells
• Platelets
• Antibodies

4. What is the normal value of MCH per cell

• 28 ± 2 picograms (pg)
• 29 ± 2 picograms (pg)
• 27 ± 2 picograms (pg)
• 19 ± 2 picograms (pg)

Blood Bank MCQs for MLT ASCP/ ASCPi

This subject describes all about the Blood product, blood group immunology, blood group systems, serologic and molecular testing, pathophysiology and physiology, transfusion practice, and serologic and molecular testing. It has 15 – 20% for MLT ASCP test.

Blood Bank MCQs for MLT ASCP

1. RBC’s optimum storage temperature is

•  -80*C
• 4*C
• -12*C
• -20*C

2. What will be the RBC phenotype of a blood donor with genotype hh, AB?

• AB
• O
• A
• B

3. Which of the red cell types are most commonly found in African Americans?

• Jk(a-b-)
• K-k-
• Lu(a-b-)
• Fy(a-b-)

4. Characteristics of anti-I is

Immunology Q&A for MLT (ASCP/ASCPi)

It is all about the immune system including diseases of the immune system, immunology principles, serology infectious disease, test results, transplantation, and serologic procedures. Immunology covers little (5-10%) part for exam questions.

Q&A for immunology

1. Phagocytosis can be defined as

• Process of engulfing foreign substances or other cells by cell.
• Movement of the cell toward any chemical response
• Process of shrinking cell
• Discharging of cells from infected body part

2. Name of the first antibody that appears after primary antigenic stimulation:

• IgG
• IgD
• IgA
• IgM

3. Inflammation of the liver is called

• Hepatitis
• Rheumatoid arthritis
• Parkinson’s and bowel disease Alzheimer’s diseases
• Inflammatory

4. Chicken pox and shingles can be caused by which virus?

• Bunyamwera virus
• Varicella-Zoster virus
• Adeno associated virus
• Aichi Virus

5. What about Rheumatoid arthritis?

Urinalysis and Body Fluids Q&A for MLT ASCP

Microscopic analysis, Chemical and physical testing, disease States, and physiology are important topics for the preparation of exam questions for this subject. It comprises the 5-10% questions for exam.

Question for this subject:

1. What is an acceptable timeframe to keep urine at room temperature?

• 2 hours
• 3 hours
• 4 hours
• Less than 4 hours

2. Normal pH value for urine is?

• 5-0-8.0
• 4.6-8.2
• 4.5-8.1
• 4.6-8.0

3. What is about specific gravity?

• The ratio of substances in urine compared to the density of water.
• The ratio of substances in blood compared to the density of water.
• The ratio of substances in urine compared to the density of other body fluids.
• None of them

4. What is the difference between gamma, alpha, and beta immunoglobulins?

• Alpha and beta globulins act as antibodies and gamma globulins act as enzymes
• No difference between them
• Alpha and beta globulins act as enzymes and gamma globulins act as antibodies
• All incorrect

5. What is pyelonephritis?

• Inflammation of the liver due to bacterial infection
• Kidney Inflammation due to bacterial infection
• Inflammation of the joints due to injury
• Bleeding of kidney

Lab Operation Q&A for MLT (ASCP/ ASCPi)

It is all about the lab operation process including safety, lab instruments, automated and manual methodology, troubleshooting, and quality assessment. 5–10% question out of 100 select from this subject.

Lab Operation Q&A

1. What anticoagulant tube is needed to collect the sample of HbA1c?

• Heparin
• Sodium fluoride
• EDTA
• Sodium Citrate

2. Which test is used to count ammonium oxalate reagents?

• Reticulocytes
• WBC’s
• Platelet
• RBC’s

3. The Widel test is performed to diagnose which bacteria?

• Proteus
• E.coli
• Shigella
• Salmonella

4. The formula of coefficient of variation is

• Mean X 100/SD
• SD X 100/ standard error
• SD X 100/ Mean
• Variance X 100/ Mean

How to Pass the MLT ASCP Exam?

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