MLS ASCP Exam Questions for Chemistry, Hematology, Microbiology, Blood Bank, Immunology, Urine & Body Fluids and Laboratory Operations

MLS ASCP Exam Questions are supportive resources for MLS ASCP certification candidates If they want to successfully pass this challenging exam. Because BOC presents an exhaustive syllabus and intricate tests that cannot be handled without any guidance.

However, BOC recommends the MLS ASCP study guide and reading list for applicants to compose exam tests, but examinees need extra information for exam preparation.

For adequate preparation, Candidates must monitor the information of other resources such as MLS ASCP exam Flashcards and MLS ASCP exam Questions. Both sources of information help to clear the candidates' blur concepts about the BOC exam pattern.

In this article, we are trying to clarify some important information about the MLS ASCP Exam, such as the exam nature, question percentage for each subject, tips to pass this exam, and some crucial questions for every exam content area.

Testing Nature of MLS ASC Exam Questions

The MLS exam is established by using the CAT (Computer Adaptive Testing) format. CAT monitors the candidate's experience by showing a series of difficult questions one by one until the candidate's mistake.

After this, it offers an easy question and then a slightly tougher level of question. In this way, CAT chooses well-experienced candidates for MLS credentials.

To achieve an MLS certificate, the examinee must answer enough questions correctly to achieve a score above the pass point. BOC does not fix any percentage to pass, nor questions limit to solve. Applicants achieve MLS credentials if they get above scores than pass points.

How Many Questions Is the MLS ASC Exam?

This exam offers 100 multiple choice questions (MCQ) with one best answer. BOC recommends a specific percentage of questions for each content area so that the examinee may get an idea about the highly focused subject for exam preparation

MLS ASCP Exam Questions Percentage

BOC selects a specific content percentage from each exam subject. This percentage is described below:

• Chemistry: 17-22%
• Microbiology: 17-22%
• Hematology: 17-22%
• Blood Banking: 17-22%
• Urinalysis and other Body Fluids: 5-10%
• Immunology: 5-10%
• Laboratory Operations: 5–10%

Chemistry Exam Questions for MLS ASCP/ ASCPi

The content area for chemistry is acid-base determinations (including blood gasses), therapeutic drug monitoring, electrolytes, Carbohydrates, heme derivatives, toxicology, endocrinology, proteins, lipids, vitamins and nutrition, enzymes, and other nitrogen-containing compounds.

Chemistry Question for Exam

1. A spectrophotometer measures the amount of photons (light)

• passes through a substance
• given off by a substance
• fluoresced by a substance
• changed by a substance

2. What is the Beer-Lambert law statement?

• The concentration of the sample and the amount of light absorbed have no relation to each other
• The concentration of the sample and the amount of light absorbed are inversely proportional to each other.
• The concentration of the sample and the amount of light absorbed are directly proportional to each other.
• All are incorrect

3. . What is carbohydrate composition?

• Hydrogen + Carbon + Nitrogen
• Hydrogen + oxygen + carbon.
• Carbon +Nitrogen + Chlorine
• Hydrogen + Fluorine + oxygen

4. Diabetes 1 is

• Patients are not able to produce insulin at all
• Patients can produce insulin, but this insulin cannot work properly
• Patients cannot produce enough insulin to work
• Patients produce more insulin than need

5. What is the purpose of the glycated hemoglobin test?

• Monitor the average amount of glucose in the patient's hemoglobin
• Monitor the total amount of glucose in the patient's hemoglobin
• Monitor the present glucose level in the patient's hemoglobin
• The glycated hemoglobin test is not for monitoring the glucose in a patient's hemoglobin 

 Microbiology Questions for MLS ASCP

Post-analytic procedures, Preanalytic Procedures; analytic procedures for mycology, parasitology, mycobacteriology, and virology; Analytic procedures for bacteriology are Microbiology content areas.

Microbiology Exam Question

1. What is another name for the disease tularemia?

• Squirrel fever
• Dog fever
• Rabbit fever
• Mouse fever

2. Which vitamin is associated with scurvy

• Vitamin C
• Vitamin A
• Vitamin D
• Vitamin E

3. How are Group B Streptococcus and Listeria similar?

• Both cause meningitis/bacteremia in women
• Both cause meningitis/bacteremia in men
• Both cause meningitis/bacteremia in old men and women
• Both cause meningitis/bacteremia in babies

4. The CAMP test is used to identify

• Group B Streptococcus
• Group C streptococcus
• Group A streptococcus
• Group G streptococcus

Hematology Questions for MLS ASCP Exam

Its specific content area is disease states, hemostasis (including disease states, laboratory determinations, and physiology), Physiology, and laboratory testing.

Hematology MLS ASCP/ ASCPi Exam Question

1. What is the hemoglobin normal range for males?

• 12.0-15.0
• 13.6-17.2
• 14.6-17.2
• 15.6-17.2

2. What % of Hgb F is seen in the body?

• 1-20%
• 1-30%
• 1-10%
• 1-25%

3. Where is Drabkin's solution used?

• It is used in the amyloglucosidase method for the determination of glycogen
• It is used in the anthrone method for the determination of glycogen
• It is used in the cyanmethemoglobin method for the determination of hemoglobin.
• It is used in the oxy-hemoglobin method for the determination of hemoglobin

4. (WBC X 100)/ (nRBC + 100) Where it is applied?

• It is used to correct a WBC count for the presence of nucleated red blood cells.
• It is used to count the correct RBC for the presence of white blood cell
• It is used to count the correct presence of WBC and RBC
• All are incorrect

5. . 2 alpha + 2 gamma chains, composition for what?

• Hemoglobin A
• Hemoglobin A2
• Hemoglobin C
• Hemoglobin F

Blood Bank Questions for MLS ASCP/ ASCPi

BB comprises content areas including pathophysiology and physiology, Blood products, transfusion practice, molecular and serologic testing, blood group systems, and Blood group immunology.

Blood Bank Exam Questions:

1. Which blood type can be used when blood type is unknown?

• O Negative
• O positive
• AB
• A positive

2. What blood groups have the least H antigen?

• O blood group
• A1 blood group
• A2 blood group
• AB blood group

3. Bombay phenotype is a deficiency of

• Antigen A
• Antigen B
• Antigen H
• Antigen AB

4. What is alpha-3-N-acetylgalactosaminyltransferase?

• Code for gene A
• Code for gene B
• Code for gene C
• Code for gene Z

Immunology Q&A for MLS (ASCP/ASCPi)

Study content for Immunology is transplantation, Principles of immunology, test results, infectious disease serology, serologic procedures, and immune system diseases.

Exam Question for Immunology

1. What are – B cells- T cells and – NK cells

• Types of lymphocytes
• Types of leukocytes
• Types of neutrophils
• Types of eosinophil

2. When latex agglutination test is done?

• Check white blood cells in the blood
• Check protein in the blood
• Check antibodies or antigens in body fluids
• Check interferon in the blood

3. Who produces Ab in the body?

• B lymphocytes
• T lymphocytes
• Natural killer cells
• None of them

4. How long do Mature T-lymphocytes survive?

• Few minutes
• Few hours
• Few days
• Months or years

5. How fast is the onset of innate immunity?

• 0-1 hours
• 0-2 hours
• 0-3 hours
• 0-4 hours

Urinalysis and Body Fluids Q&A

The content area for this subject is Physical and chemical testing, disease States, physiology, and microscopic analysis.

 Exam Question for Urinalysis and Body Fluids

1. Normal urine color is

• Red or pink
• Red and dark brown
• Clear to pale yellow
• Light red

2. . How many bacteria must be found in urine to indicate a UTI?

• > up to 100,000 colonies of bacteria per mL
• > up to 100,00 colonies of bacteria per mL
• > up to 100,000,0 colonies of bacteria per mL
• > up to 100,0 colonies of bacteria per mL

3. Which test is performed to monitor kidney performance?

• Urine albumin-to-creatinine ratio (UACR) test
• Glomerular Filtration Rate test
• Serum creatinine test
• Blood urea nitrogen (BUN) test

4. The creatinine clearance is reported in

• ML/24 hours
• Mg/24 hours
• Mg/dL
• ML/min

Lab Operation Q&A for MLS (ASCP/ ASCPi)

Laboratory Operation study content is education principles, Quality assessment/troubleshooting, instrumentation and manual/automated methodology, safety, basic management principles, laboratory mathematics, and Quality assessment/troubleshooting.

Exam question for Lab Operation

1. What is the Recognition site and cleavage site for EcoR1?

• (5′-ALAGCTT-3′ 3′-TTCGA A-5
• (5-GIGATCC-3′ 3-CCTAG G-5 )
• (5′ G|AATTC 3′, 3′ CTTAA|G 5′)
•  (5′-GTCGAC-3′ 3′-CAGCTG-5′)

2. Which test is performed to detect the presence of serum antibodies against HIV protein antigens?

• ELISA-HIV Test
• p24 antigen test
• HIV antibody/antigen tests
• None of them

3. Which influenza only attack human not animal? 

• Influenza A
• Influenza B
• Influenza C
• All

4. 5-GIGATCC-3′ 3-CCTAG G-5 Recognition site and cleavage site for

• Bam HI
• Sal I
• Bal 1
• Hae III

How to Pass MLS ASCP Exam?

The American Society of Clinical Pathology (ASCP) offers MLS credentials for those who are applicable for it by checking hir/her skill and knowledge of relevant content areas.

Achieving an MLS certificate is not for everyone, but for those with firm determination and tricks to pass this exam. To help the examinee, here we describe the essential magical tricks to pass this exam.

FAQ for MLS ASCP Questions