MLS ASCP Study Guide, Material and Exam Questions for ASCP/ ASCPi

MLS ASCP Study Guide for Success

Study for MLS ASCP exam is the most critical step in your certification journey by the BOC.

The medical laboratory scientist exam is all about testing your theoretical knowledge of your expertise and skills as a medical laboratory scientist.

Passing this exam grants you the highest credential in the field of medical lab science as MLS ASCP or MLS ASCPi.

Start your MLS ASCP study journey with an A-Z guide today. Believe in your abilities and work with continuous effort and focus.

MLS ASCP Study: What is All About?

It is all about preparation for MLS ASCP certification by ASCP BOC to get certified as MLS ASCP or international MLSi.

MLS ASCP Study Reading List and Preparation Bundle by MLSIA

You need a very conclusive and comprehensive one unified study material instead of the 49 reading sources recommended by the BOC.

Also, you need Q&A to train yourself on how to think, process and answer questions in your real exam.

The Importance of MLS ASCP Study

MLS ASCP Learning plays an important role in the foundation of medical diagnosis.

Additionally, MLS study provides opportunities to develop practical skills through clinical practice.

Hands-on experience in an actual laboratory environment leads to improved clinical judgment and troubleshooting skills.

In addition, since knowledge and information are shared within the learning community, it is possible to build a professional network and promote continuous learning.

MLS ASCP Study Guide for Effective Exam Preparation

Our MLS ASCP Study Guide provides study content and a syllabus for certification as a Medical Laboratory Scientist.

Below is a brief description of the main content and syllabus of the MLS ASCP study guide according to the BOC.

ASCP/ ASCPi Study Guide/ Material for MLS

Blood Banking

Blood Banking covers blood product handling, cross-type glycemic reactions, transfusion reactions, antibody screening, and more.


Chemistry covers content related to serum and plasma biochemical tests, enzymatic reactions, electrolyte balance, metabolic panels, and toxicology.


Hematology covers content related to the morphological evaluation of blood cells, pathological changes in hematopoietic tissue, anemia, and blood clotting disorders.


Immunology covers topics related to detecting immune responses, autoimmune diseases, tumor markers, and infectious diseases.

Laboratory Operation

Laboratory operations cover content related to quality control, quality assurance, safety procedures, laboratory efficiency, and legal and ethical issues.


Microbiology content is related to identifying microorganisms, diagnosing infectious diseases, bacteriological and mycological testing, and infection control.

Urinalysis and Body Fluids

It covers chemical and microbiological analysis of urine, cytological evaluation of body fluids, and detection of abnormal constituents.

MLS ASCP Study Material

Having relevant study materials with good resources for learners pursuing the MLS (ASCP) credential is important. Our learning material includes:

Study Guide

Our study guide provides an overview and goals of the MLS ASCP program and guides learners on how to proceed. The guide includes a detailed curriculum for each subject, how to proceed with learning, evaluation methods, etc.

One Unified Study Material

Your success actually depends on having an all-in-one conclusive study material that covers all the subject areas of the ASCP exam.

Having one study sources is super crucial to save time, money and effort during your preparation phase of MLS certification.

The 4×4 MLS ASCP study material is the comprehensive conclusive juice of the reading list recommended by BOC that has more than 49 resources between text book, journals and online resources.

MLS ASCP Study Reading List and Preparation Bundle by MLSIA

The ASCP BOC Reading List For MLS Exam

ASCP has provided a reference reading list for MLS study. Below are general texts and reference guides for each subject of MLS certificate exam.


Blood Banking has 3 text books and 4 online resources


Chemistry has 2 text books and 3 online resources


Hematology has 2 text books and 4 online resources


Immunology has 2 text books

Laboratory Operation

Laboratory Operation has 6 textbooks


Microbiology has 10 text books and 4 online resources

Urinalysis and body fluids

Urinalysis and body fluids has 4 text books

If you want a detailed guide, you can visit our post here or the official website of ASCP.

Understanding Important Topics MLS ASCP Study

The BOC exam requires an understanding of key topics in each area. Get to know the key topics in each subject area:

Blood Banking

  • Fundamental Principles and Techniques of Immunohematology
  • Indications for blood transfusion and management of abnormal reactions
  • Immunological test methods and their interpretation


  • Interpreting biochemical panels and identifying outliers
  • Blood gas and electrolyte analysis
  • Understanding Drug Audits and Drug Metabolism


  • Features of erythrocytes and leukocytes
  • Evaluation of blood clotting mechanisms and clotting factors
  • Diagnosis of anemia and leukemia


  • Basic concepts and functions of the immune system
  • Evaluation of Immunoglobulin and immune response
  • Diagnostics for autoimmune diseases

Laboratory Operation

  • Principles of Quality Control and Quality Assurance
  • Workflow and laboratory efficiency
  • Information management and data analysis


  • Characterization and identification of bacteria, viruses, and fungi
  • How to perform antibiotic susceptibility testing
  • Fundamental Principles of Infectious Disease Diagnosis and Infection Control

Urinary Fluid Testing and Body Fluids

  • Basic Concepts and Methods of urinary fluid testing
  • Analysis of body fluids (cerebrospinal fluid, synovial fluid, pleural effusion, etc.)
  • Chemistry of tumor markers and body fluids

MLS ASCP Study Questions

Distribution of MLS exam question per each subject area according ASCP BOC:

Blood Banking: 17-22%

Chemistry: 17-22%

Hematology: 17-22%

Immunology: 5-10%

Laboratory operation: 5-10%

Microbiology: 17-22%

Urinalysis and body fluids: 5-10%

MLS ASCP Study Guide Exam Questions Percentage
Credit: ASCP BOC

MLS ASCP Study Question Examples

The minimum hemoglobin concentration in a fingerstick from a male blood donor is:

  • 12.0 g/dL (120 g/L)
  • 12.5 g/dL (125 g/L)
  • 13.5 g/dL (135 g/L)
  • 15.0 g/dL (150 g/L)

A cause for permanent deferral of blood donation is:

  • diabetes
  • residence in an endemic malaria region
  • history of jaundice of uncertain cause
  • history of therapeutic rabies vaccine

Which of the following prospective donors would be accepted for donation?

  • 32-year-old woman who received a transfusion in a complicated delivery 5 months previously
  • 19-year-old sailor who has been stateside for 9 months and stopped taking his anti-malarial medication 9 months previously
  • 22-year-old college student who has a temperature of 99.2°F (37.3°C) and states that he feels well, but is nervous about donating
  • 45-year-old woman who has just recovered from a bladder infection and is still taking antibiotics

Which one of the following constitutes permanent rejection status of a donor?

  • a tattoo 5 months previously
  • recent close contact with a patient with viral hepatitis
  • 2 units of blood transfused 4 months previously
  • confirmed positive test for HBsAg 10 years previously

According to AABB standards, which of the following donors may be accepted as a blood donor?

  • traveled to an area endemic for malaria 9 months previously
  • spontaneous abortion at 2 months of pregnancy, 3 months previously
  • resides with a known hepatitis patient
  • received a blood transfusion 22 weeks previously

How To Study for MLS ASCP?

Success requires good preparation and effective study strategies. Here is how to study for ASCP/ ASCPi MLS exam.

Total Time: 1 minute

Create a Study Plan

An effective study plan is critical to preparing for the MLS ASCP study exam.

Goal Setting

First, set goals that you want to achieve before the exam date. Setting specific, achievable goals can help keep you motivated.

Gathering Study Materials

Collect study materials related to the MLS ASCP exam. It is important to cover a wide range of knowledge by utilizing the official ASCP guidelines, textbooks, past exam papers, etc.

Creating a Schedule

Plan your daily study hours and study areas to ensure overall exam coverage.

Adopt Active Learning Methods

The MLS ASCP exam requires not only memorization of information, but also application and analytical skills. You can deepen your understanding by solving workbooks and practice tests, and participating in group discussions and debates.

Practice Questions and Mock Tests

It is also important to include practice questions and mock tests in your study plan. Check your understanding of past exam questions and online resources.
You need to focus on areas where you have gone wrong or where you have weaknesses to deepen your understanding. We provide a large question bank to practice from. You can subscribe to the MLS ASCP Study Exam Prep Course.ASCP/ ASCPi Study Guide, Material and Q&A for MLS

Get the Ultimate Solution

Subscribe to the All-in-one inclusive MLS ASCP Prep Package. Live online classes, one unified study material and Q&A at MLSIACADEMY.

FAQ for MLS ASCP Study

How do I pass the ASCP MLS exam?

To pass the ASCP MLS exam, preparing and focusing on key topics is important. Solve and practice past exam questions and mock exams. Use guidebooks and study materials to thoroughly study the content of the exam.

What is a passing score for the ASCP MLS exam?

A passing score for the ASCP MLS exam is considered to be 400 or higher out of 999.

How hard is ASCP?

It is very difficult. The exam covers a wide range and requires specialized knowledge and skills, so sufficient preparation and study are required.

What is the difference between ASCP MLS and ASCP MLT?

ASCP MLS (Medical Laboratory Scientist) and ASCP MLT (Medical Laboratory Technician) are professions with different roles and responsibilities.


MLS ASCP for US candidate while ASCPi for the international non-us candidate

What is the highest score for the ASCP?

The maximum score for the ASCP exam is generally 999 points. The highest possible score is obtained by answering all the questions on the exam correctly and getting a perfect score.

What is the validity of ASCP?

ASCP certifications have a fixed validity period. ASCP certification is valid for three years. To maintain certification, you must complete the recertification process before expiration.

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